FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509638
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC15150.061.690733
AGCGAAA16100.056.7652851
GCGAAAG18250.050.0400352
GAGCGAA8350.023.4539171
TAGTACC13150.023.1395024
GTATACG3655.666152E-921.8593861
AATACCG1750.003675585820.699625
TCTATAC29950.020.561983
CGTTACC1850.00506965519.5810017
TATACCA42300.018.4975345
CTATACC27200.018.3785254
TATAATA36450.017.6971112
CTACGGA2901.8767834E-417.488113
GTATATA34900.017.2500671
TCGTACC2600.001821562316.7189244
TATACGG4851.487133E-716.438452
GTCCTAT27550.016.3233091
CGGTAGT2700.002343787316.0999366
GTATAAT20850.016.0025751
CTCTACG4108.998808E-615.9219691
Produced by FastQC (version 0.11.5)