FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546008
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA19500.044.641981
CGAAAGC20650.044.563993
GCGAAAG21450.041.228582
GTGATCG5350.024.3791839
ATATCGT4505.456968E-1222.5409956
TATATAC25250.022.096823
TATATCG3955.966285E-1022.0110995
TATACTG17800.019.9449835
TTATACT19450.018.2524764
GTATATA23100.018.2143141
TCTATAC24900.017.7513313
GTATTAT32050.016.9757921
GTCCTAT26200.016.8898321
CGGTTAC6104.311005E-1016.6286036
TTATATA22450.016.4671962
CCGGTTA6607.4578566E-1116.4666355
CTATACC25550.016.163274
GTATTAG18700.015.9051971
TATACTA10400.015.3266375
GTCCGAA3406.242445E-414.9353691
Produced by FastQC (version 0.11.5)