FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3346817
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG16200.054.146362
AGCGAAA16600.051.5769921
CGAAAGC17700.050.353493
GAGCGAA7900.023.8796711
GCTATAT16200.021.0506231
TATATAC29700.020.9816553
ATATCGT7950.018.2289076
TATACCA38000.017.7333415
CTATATA21600.017.4521332
GTATATA24550.016.5507851
GTATAAG10150.016.4415931
TATATCG4901.6763988E-716.2662645
GTATAAT18250.015.9030061
TATAGGT14700.015.2802024
AAAGCAG116750.015.0813655
TATACTA10100.015.0656965
GTCTAGA22800.014.9570221
CTAGCAC17600.014.8213433
GTATTAA28300.014.6140481
GTGCTAG17050.014.4689371
Produced by FastQC (version 0.11.5)