FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114111
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA24400.061.835831
CGAAAGC27050.056.2499663
GCGAAAG29300.051.2047462
TATATAC24450.021.0401953
GTATATA23750.019.5471341
GAGCGAA13800.019.4486451
TATACTG32200.018.6765985
TTTATAC27850.017.9512273
TATACCA34900.017.4393145
GTATAAA26650.017.1478651
TTATACT17900.016.1909354
AAAGCAG145400.015.9463435
CTAGTAC7803.6379788E-1215.7915433
GAAAGCA105700.015.6973194
GTATAGG14150.014.8664851
CTATACC27350.014.8352784
TCTATAC27100.014.7049543
GTATACT14950.014.5393514
ATATACC25750.014.3501984
TAGTATA11750.014.1872252
Produced by FastQC (version 0.11.5)