FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018386
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4700.060.1912161
CGAAAGC5000.057.9535643
GCGAAAG5200.054.3635062
CCGAGCG950.00474361130.5048719
TGATCCG2553.0468073E-928.4114049
GAGCGAA2404.345333E-521.1569541
CCGACGC2404.3700977E-521.141361145
GTATAGG4202.8396244E-818.9980831
GTATAGA7800.018.5995221
GTATAAG3151.6759686E-518.4223821
GTGCTAG3002.4072388E-416.9255641
GAGTGCG2600.001820296416.7190199
TATACTG9700.016.4301365
GGTATAC2700.002323254216.1195851
GTTATAG4551.3833342E-615.921313
TAGGACT7402.5465852E-1115.6615894
AAAGCAG29700.015.6103885
ATAATAC9750.015.6028853
AAGCAGG37600.014.8351896
GCCTAAG3951.16400144E-414.6912671
Produced by FastQC (version 0.11.5)