FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561678
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG41400.161612817848301No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA41350.1614176332856823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC15350.047.6724363
AGCGAAA16350.044.3689841
GCGAAAG17000.043.0681082
GAGCGAA9700.034.4019741
AAGGTCG5700.029.2369989
TATACTA27250.028.996352
ATTATAT26300.028.962151
GAGCAAA60900.027.3973031
GTATTAA34450.026.3219491
TACTAGA30150.025.7133884
TTATATT29850.025.2564532
CAAAAGC63800.025.2112944
TATATCG2051.29784075E-524.7399355
TATTAAT31700.024.6972052
ATACTAG32200.024.3008943
CCGACGC8450.023.159595145
GCAAAAG67600.023.1505553
ATATTCA32750.022.3446164
ATTCAGC31150.022.328916
GTATACT35600.022.2112881
Produced by FastQC (version 0.11.5)