FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448062
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA14000.038.8710141
GCGAAAG14200.038.799582
CGAAAGC15550.036.809963
GAGCGAA7800.026.9771461
GAGCAAA65250.025.2428251
CAAAAGC64900.024.112724
GCAAAAG69850.022.716763
TAGTACC12300.018.8487284
GTATTAT29100.017.9527971
TATACTG22600.017.6315885
GTATATA24050.016.8952871
TATATAC22650.016.6342723
TTATACT24250.016.4319134
AGCAAAA101150.015.9823542
GTATTAG21850.015.9397851
GCTATAT12800.015.8723311
AGACGGT7955.456968E-1215.499378145
GTGTCGA4251.2773549E-515.3443139
AAAGCAG120850.014.5083676
AAAAGCA113850.013.8726885
Produced by FastQC (version 0.11.5)