FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822003
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC20150.042.4326933
GCGAAAG21550.039.355772
AGCGAAA24650.034.43251
GAGCAAA80650.032.381561
CAAAAGC92400.028.229024
GCAAAAG100400.025.47623
GAGCGAA18300.022.9919911
TATATAC24900.022.6980693
TGACGCG2953.8895996E-722.113146145
AGCAAAA118850.021.347092
AAAGCAG147350.020.3581456
GTATATA21850.018.2604261
AAGCAGG162050.018.0642667
AAAAGCA145700.017.9520025
TAGTACC16600.017.8953844
GTATTAT30700.017.0135061
TTATACT18600.016.7502294
CTATATA15850.016.4642932
GTGATCG6901.4551915E-1015.7524179
GATAGCG6459.458745E-1015.732523145
Produced by FastQC (version 0.11.5)