FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485381
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA15550.050.8569071
GCGAAAG17150.046.497882
CGAAAGC21350.038.0134933
TGATCCG5900.027.0201849
ACGCGCC6150.022.386378
TACGAAC2303.1572563E-522.0540249
GAGCGAA8700.021.6824281
GTATATA39200.021.2846051
TATACGA2857.1393097E-620.339977
CGCGCCA6650.019.6141059
CCGACGC5901.2732926E-1118.427876145
GACGCGC5158.1308826E-1018.2911627
TAGACCG2000.0079457518.1147675
GTATTAA32550.018.05461
TAAGACG5301.1914381E-917.7727224
ATAGTAC24400.017.2248653
TAGTACC21850.016.91244
TATACCG3554.7273323E-516.3288025
GTATAGG13150.016.0002251
AGACGCG5904.951289E-915.9655556
Produced by FastQC (version 0.11.5)