FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878617
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA20350.049.2097931
CGAAAGC21250.047.0560383
GCGAAAG21450.046.6391562
ACGCGCC11350.030.645258
CGCGCCA13000.026.7561289
AACGCGC5600.025.8792827
TATTCGT2755.2384457E-621.0805859
AAACGCG7250.020.9878986
TAAGACG10500.020.7023494
ATATTCG2908.294597E-619.9898628
GACGCGC10550.019.9184637
AGACGCG10450.019.414646
GTATATA18250.019.0860141
TACCGTA7750.018.7001958
GTAGTAA27100.017.6730521
GTATAGA18150.017.1920911
TGATCCG10300.016.8849339
AAGACGC12200.016.6297555
GTATACG3103.084569E-416.3860111
GAGCGAA13050.016.1259161
Produced by FastQC (version 0.11.5)