FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341340
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT138530.41459414486403656No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG94050.28147389969293757No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT83200.2490018974423435No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA82240.24612879862570106No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC79110.2367612993589398No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC73530.22006141248720573No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC71380.21362686826243363No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT66460.1989022368271412No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA63430.18983401868711355No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA60530.18115486601183955No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA53250.1593671999856345No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG53220.1592774156476144No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG53190.15918763130959435No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG52400.15682331040839903No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG50270.15044862240897366No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT50100.14993984449352657No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC48780.1459893336206432No Hit
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG48760.1459294773952965No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT47220.14132054804359928No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT47100.140961410691519No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC47020.14072198579013212No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC46330.13865694601567036No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA45160.1351553568328874No Hit
GTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGG43720.13084570860792377No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG42140.1261170668055331No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA41930.1254885764393926No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT41420.1239622426930513No Hit
GCCTGATACAGATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAA41300.12360310534097099No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT40190.12028108483422817No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC39530.11830582939778651No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG38760.11600136472193791No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA37920.11348740325737579No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC37670.11273920044054181No Hit
TCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT37540.11235013497578816No Hit
ACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTG37370.11184135706034107No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT34940.10456882568071492No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT34920.10450896945536821No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT34290.1026234983569466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC20150.060.0547873
GCGAAAG23850.051.368052
GAGCAAA51050.045.195681
GTCATAG34750.041.9310722
CAAAAGC56050.040.5895964
AGCGAAA30050.040.080131
CATAGTG40500.035.6007164
TGATCCG40050.035.2781529
GGTCATA48350.031.662961
GTATTAA84600.031.1316261
TCATAGT47600.030.5981623
ATAGTGA48900.029.1889235
TTAATTG85150.028.2497184
TAGTGAT51850.027.3884856
TAATTGA88450.027.3595735
TTGATAT90150.026.8444448
ATTAATT92150.026.8142243
TATTAAT100400.026.5710562
ATGTCCG11950.026.0719959
GCAGGTC34950.025.706819
Produced by FastQC (version 0.11.5)