FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4476486
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT164270.36696194291683254No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG115350.2576797961615428No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT109380.24434344260207672No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC108050.24137236216085564No Hit
CTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGA106190.2372173173332833No Hit
GTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGC92480.20659061594295167No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC87990.1965604270849948No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA85530.19106504521627007No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT74720.16691663952484156No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA73760.1647721002589978No Hit
GATTAGATTAACCGGCGACAAGCGGGTTAATGAAACAGAATTTGCCTGGC71760.16030431012182322No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT65230.14571697532394828No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG65180.1456052805705189No Hit
GACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCG65150.14553826371846132No Hit
CTCTAAGTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATG64800.14475640044445576No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA64080.1431479959950729No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT62160.13885891746338533No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT61830.13812173209075154No Hit
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG60210.13450282207964015No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA59170.1321795712083094No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA56500.12621507137518134No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC55900.12487473433402897No Hit
TCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAATG54940.12273019506818517No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG54920.12268551716681343No Hit
GCCTTATTGACCGGGGTTTTTCTAATTTGATGCCTGGCAGTTCCCTACTC52580.1174582027063192No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT51790.11569342560213525No Hit
CCCTGAGACTTAGAGTCTCCTGAAGGAACGTTGAAGACGACGACGTTGAT51120.11419671590618177No Hit
GATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCT48690.10876835088951468No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC47150.10532815248389027No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG46010.10278151210570076No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT45580.10182093722620825No Hit
GTCTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCA45560.10177625932483648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC41300.056.323113
GAGCAAA68050.050.4333761
GCGAAAG46450.050.0974852
AGCGAAA50650.045.4113921
CATAGTG46950.043.6755944
CAAAAGC77500.042.446524
GTCATAG49450.041.0476572
TGATCCG52350.038.343769
TCATAGT54000.038.1115153
GGTCATA61850.034.3726461
GTATTAA111050.032.0810051
TTACGAT15500.030.8531994
GCAGGTC43300.029.6221799
ATGTCCG16400.029.604839
ATAGTGA70050.028.9626225
TTTACGA16900.028.7289473
TAGTGAT70950.028.5955496
ATTAATT110200.028.4733073
TAATTGA105650.028.4620745
TTAATTG106350.028.2747384
Produced by FastQC (version 0.11.5)