FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3929607
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC13500.059.0391773
GCGAAAG15800.049.539672
AGCGAAA16600.047.189451
GTCGTAC7900.033.0525251
CGTACTC8350.032.106773
TCGTACT8900.029.3156092
TACGGAT9550.027.3203052
TGACGCG1701.03333434E-425.583538145
ATGCGTC10900.024.605574145
GTACGGA11700.024.1773131
ACGGATA11800.022.105583
TCGTGCG2353.7275036E-521.5846379
GGTACTG17400.020.4043485
CGGATAA12950.020.1422794
GTACTGA23550.019.3834486
CAACGCG1950.006867414318.5787643
TACTGAT29000.017.9898267
CAGTACG10100.017.9363149
ACGTATG9450.017.642263145
AGGTACT21800.017.615514
Produced by FastQC (version 0.11.5)