FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160342
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA25100.058.3896751
CGAAAGC28000.052.7924233
GCGAAAG29500.050.1309662
TATAGCG1958.7694625E-626.0232372
GTATATA27300.022.0583931
TAGTCGC3055.3750045E-721.3824659
ATAGTCG3406.481241E-821.311828
GTATAAG10350.021.0300031
GTGATCG7350.020.7036579
CCGTATA2359.225114E-418.505144145
ATAATAC33500.017.7365233
TATAATA34800.017.7066972
ATTACCG3302.5281895E-517.565055
TACCGTC2952.1367134E-417.1939437
TATATCG2600.001820434616.7205775
GAGCAAA63800.016.7168731
TAGAGTG26300.016.5298485
TATACTG27300.016.4551725
GTATAGC11100.016.340881
GTATAAT29000.016.0118081
Produced by FastQC (version 0.11.5)