FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256216
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5150.063.300893
GCGAAAG5700.058.487152
AGCGAAA6050.055.1409151
ATGCGCG553.230276E-452.6909987
CGCACCG900.003641540732.1987725
TAACGAC1751.2625408E-424.8380643
GCCGACC1550.0018113823.381247145
GTATATA11700.022.314551
GTGTACG2205.8922986E-419.7788091
CACCGTA1900.00591216519.0658237
TGCCCGA1950.0068703118.5762186
GCTATTG6251.8189894E-1218.548718
TATAGAC7050.018.4964313
GACGTAG2000.00794864918.112537
TACATCG2400.00106510818.1118095
AAGACCG3653.0070532E-617.8637055
GAACCGC2550.001599074117.0464086
TATACTG9100.016.7185945
CTATTGA6959.094947E-1216.6811569
TATATAG6104.311005E-1016.6265743
Produced by FastQC (version 0.11.5)