FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766367
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG18150.059.5075572
AGCGAAA18650.058.3504451
CGAAAGC19500.055.3667833
TATACCG1301.6625292E-533.4418145
GAGCGAA7500.025.1503371
GTATATA21050.021.7130181
GACGTAT4358.54925E-1121.655076
GTATAAG11550.021.3564321
TATATAG11850.018.9629482
TAGTATA7700.018.8278962
GAAAGCA62250.018.5071564
ATACCGT2751.2483184E-418.4446666
CGTATGC4455.583388E-817.9117558
AAAGCAG97850.017.1053925
TATACTG11150.016.8958645
ACGTATG5703.1322998E-916.5262367
GTATTAT15500.016.3820461
GTATACT7201.6370905E-1116.1016144
ATTATAG19400.016.0606963
TATATAC21250.016.026423
Produced by FastQC (version 0.11.5)