FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265123
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA15000.054.155651
GCGAAAG15400.052.711032
CGAAAGC16400.049.9212883
GAGCGAA9950.025.5130271
TTCGACC2908.275432E-619.995369145
GTATAGG8450.018.025181
TCTATAC22250.015.955753
GTATATA17150.015.6478471
GTATAGA21900.015.5657761
TAAACCG3305.027047E-415.3674575
TATAATA25050.015.3345952
TGACGCG2850.003350550515.2596245145
TATAGGT9050.015.2093154
CTATACC21000.015.178824
GTATAAT12500.015.0862171
GTCTAAC8801.8189894E-1214.8356581
GTTACGT4902.9905914E-614.7866028
AGTACGG3457.0437655E-414.6993075
GCGACCG3507.8250025E-414.496643145
GCGCACG4051.4507002E-414.317671145
Produced by FastQC (version 0.11.5)