FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513032
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG20300.054.2655032
AGCGAAA21200.051.3051031
CGAAAGC23750.046.0638053
GTATATA27150.026.9747051
GAGCGAA13600.023.4595091
CGATACA2802.3509892E-723.298546145
CGACGAA2053.6743208E-421.214937145
TATATAC32700.018.832923
ATACCGT6807.2759576E-1217.046896
ATATTCG3504.1883563E-516.561258
TATACTG23600.015.9631755
GTATTAG18350.015.8062581
GTATAGG7352.1827873E-1115.7847541
GTATTAT36050.015.6889711
GTGATCG9600.015.0951059
ATAATAC35300.014.9828583
CTGATCG10250.014.8447489
TATACCG3457.0427236E-414.6996465
GTATAGA30100.014.6949631
CTTATCG12850.014.6594163
Produced by FastQC (version 0.11.5)