FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754881
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC8450.066.8793643
GCGAAAG8900.061.897892
AGCGAAA9200.059.927321
TGACGCG1204.0653368E-430.20065145
GAGCGAA4300.026.9929661
TATATCG2104.3358246E-420.7001655
GTATTAC8250.018.4656431
GCCGACC3002.422879E-416.912363145
GTCTAAT8150.016.0218981
ACCATGT13250.015.8584638
GTATAAG3706.6650144E-515.68510151
GTATTAG8350.015.6381391
AAAGCAG65000.015.1588915
AAGCAGG76350.014.7092076
GAAAGCA43750.014.4064944
GTATAGA12800.014.1686721
GTATAGC3609.6203166E-414.10571
GTATATA12900.014.0588371
GTCTTAG12700.013.7090261
GTATAGG4252.1677642E-413.6552661
Produced by FastQC (version 0.11.5)