FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2603_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2603_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028721
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC26750.053.0923653
AGCGAAA27750.050.450881
GCGAAAG29650.047.427232
GTATATA25550.024.700331
GAGCGAA17250.016.8207471
GTATTAG17650.016.439541
TATACGA3554.726964E-516.329077
GTATAAA26250.016.304111
TAGTACC22200.015.6664964
GTCCTAT26100.015.5640251
ATTATAC20150.015.462983
AAAGCAG154700.015.4563625
ATAGTAC25100.015.3003713
GTATTAT36500.015.104111
CCTATAC17800.014.65488153
AAGCAGG162600.014.5273396
TCTATAC38350.014.3597883
CTATACC40150.014.0764354
GAGCAAA66050.013.9477861
TATACCA40200.013.6984425
Produced by FastQC (version 0.11.5)