FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491037
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA4750.077.874461
GAGCAAA38600.048.6665951
CAAAAGC40350.045.6004874
AGCGAAA8650.042.7318652
GCAAAAG44050.041.279673
GTTACGT1859.640644E-1139.205481
GCGAAAG9550.038.704782
CGAAAGC10000.037.6688734
TCTACGC1406.6292523E-736.2382972
AGCAAAA49900.036.020432
AAAGCAG70500.031.443166
CAATAGG10050.030.31111
GTCTACG1204.049121E-430.220891
AAAAGCA61600.029.6346045
CTACGCC2354.332469E-827.7448923
TTACGTG2654.5783963E-927.349662
AAGCAGG83450.026.8241527
TACGTGT3152.890556E-822.9984113
ATAGGAG13700.022.738213
GCTATTG8000.022.6398228
Produced by FastQC (version 0.11.5)