FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269437
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA80750.047.9643971
GAGCGAA11850.047.741551
CGAAAGC23250.041.763493
AGCGAAA22900.041.174481
GCGAAAG23850.040.1180652
CAAAAGC105450.036.145494
CGACGAA2154.8748916E-1033.715984145
TATACCG900.003638294832.2061655
GCAAAAG117550.031.808413
AGCAAAA130850.028.6398832
AAAGCAG151900.028.3380646
ACTCCGA7350.027.630641
ACATGGT23300.027.368335
AAGCAGG157150.027.2530177
TAATCGT4950.026.352929
GTTACGT5550.026.137091
GCTATAT7100.025.5388851
AAAAGCA155850.024.3638575
TATATAC17950.020.9919993
GTTGACA32950.020.9116571
Produced by FastQC (version 0.11.5)