FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078823
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6700.061.7081451
AGCGAAA11300.047.5002331
GCGAAAG12400.043.8387222
GAGCAAA48250.043.294991
CGAAAGC13350.042.325473
CAAAAGC52650.039.761894
GCAAAAG59750.034.6750073
AGCAAAA73800.027.9902532
AAAGCAG92050.026.5999836
AAAAGCA84750.024.4458075
GCGATTA1851.8347155E-423.5076145
AAGCAGG105300.023.3222377
GCCGACC1952.620514E-422.302084145
GCTATAT6950.020.8731351
CTATATA9200.020.4834882
GTAGAAA16500.020.2216381
TATATAC15250.019.476123
GTTACGT2256.8493513E-419.3424381
GAATCGC1900.00587397919.0879331
ACATGGT10400.018.1099835
Produced by FastQC (version 0.11.5)