FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071996
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA76100.048.6158331
GAGCGAA14050.041.8216251
CGAAAGC23300.038.564183
AGCGAAA23650.037.1147421
CAAAAGC102350.036.2481884
ACTCCGA8250.036.05141
GCGAAAG24950.035.732712
GCAAAAG110450.033.3285453
TATATCG1453.5051955E-529.9843395
AAAGCAG142600.029.5747956
AGCAAAA124800.029.4458982
AAGCAGG145900.028.9063387
GTTACGT4900.028.1287041
TAGTACC16250.026.7548224
AAAAGCA145050.025.7776435
GATAGCG6900.025.212437145
GAATCGC2601.1398879E-725.1108461
GTCGTCT4053.274181E-1123.267033145
AATCGCT2953.888963E-722.1132182
AGTACCT18250.021.4408575
Produced by FastQC (version 0.11.5)