FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119543
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA268280.8599977624927754No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG225740.7236316345054388No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC199630.6399334774356372No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG149960.48071143754069107No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA123580.3961477690802787No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG105180.33716477060902833No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG99720.31966220693223335No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA92940.2979282542346748No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG90440.2899142598771679No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT87870.28167587367765085No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT86110.27603402164996604No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC83340.26715451590184847No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG81990.26282695894879476No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA76770.24609373873032045No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG76240.24439477192652898No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT75390.24167001384497663No Hit
ATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTC72800.23336751569059955No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA70290.22532146535566266No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC67530.21647401558497512No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT67490.216345791675255No Hit
CCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCT67000.21477504878118364No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC65290.20929347664064898No Hit
GTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAAT65070.20858824513718838No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA63770.2044209680712848No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC63330.20301050506436358No Hit
GCTTAATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAATGAGGAACCCT63170.20249760942548317No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC59950.19217558469301432No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC59870.19191913687357412No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC58000.185924669094159No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG57920.18566822127471877No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG57470.18422570229036755No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC56340.18060337684077443No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG53790.17242910259611743No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA52780.16919144887568466No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA52620.16867855323680422No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA51280.16438305226118055No Hit
GTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAATGGA51000.1634854848931398No Hit
TAACAAGACCACTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTT50710.16255586154766902No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG50180.16085689474387754No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA47730.15300318027352083No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT46560.14925263091420762No Hit
CTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGGTC46060.14764983204270626No Hit
ACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG44860.14380311475110297No Hit
CTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTTTAATTGAAGAC44190.14165536426329114No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA43760.14027695723379996No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT43000.13784070294911788No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA41950.13447482531896499No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC41790.13396192968008455No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG41420.13277585851517354No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT41240.13219885092143305No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG40680.13040371618535151No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG39860.12777512603608926No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG38690.12402457667677605No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT38140.12226149791812456No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC38010.12184477021153418No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG37860.12136393055008378No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTCTTCTGTCTTCTT37180.11918412408484193No Hit
GAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT36840.11809422085222099No Hit
ATGCTGGTCCGTCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAG36520.1170684295744601No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG36490.11697226164217002No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC36380.11661964589043972No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA35560.11399105574117747No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT35230.11293320848598656No Hit
GGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC35090.11248442480196619No Hit
TTCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAATGCTGG34380.11020845040443425No Hit
CAATTAAAGTGCCGGCATCTTTTCCAAGAATTGTTAGCCTTTTAGTGGTC34350.11011228247214415No Hit
AAAGAGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAAT33580.10764397221003205No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCT32390.10382931089585878No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC31970.10248295984379764No Hit
CTTTAATTGAAGACCCAGATGAAAGCACATCCGGAGTGGAGTCCGCCGTC31780.1018738962726271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8500.0104.955431
AGCGAAA19150.079.915751
AAGCAGG135850.062.726137
AGCAGGT112150.062.608358
GCAAAAG122650.062.2122043
CAAAAGC124600.061.814194
CGAAAGC26450.057.8058173
GCGAAAG26900.057.3950422
TTTAAAC44200.056.2323953
ACTATTC45000.054.1046458
GCAGGTC70950.053.824499
GTAGAAA38550.053.4333841
TAAACTA46950.052.9361535
AAACTAT47300.052.085716
GCAGGTA61400.051.456459
CTATTCA47550.051.2047779
AAGGTCG19600.049.1719369
AGCAAAA162900.048.723682
TAGAAAC42500.048.0958062
GTATTAA88400.046.4390031
Produced by FastQC (version 0.11.5)