FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795094
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9050.063.3182261
GAGCAAA68350.048.604581
CAAAAGC75150.043.2851524
GCAAAAG76450.041.9820253
AGCGAAA19250.041.072011
GCGAAAG20700.038.8700522
CGAAAGC20700.038.8498883
TATCGTG2501.8189894E-1234.77597
AGCAAAA100400.031.9839842
AAAAGCA108650.030.2063225
GCTATAT10500.029.7049881
AAAGCAG128550.029.5322366
TATATCG2603.754394E-927.8648075
CTATATA13500.027.3841292
AAGCAGG154500.025.0877917
GCCGACC3001.7147613E-824.160305145
CTAGTAC4550.023.8845463
GTTACGT3402.4956535E-923.4673481
TATATAC20100.022.7081453
ATCGTGG3604.8930815E-922.1386248
Produced by FastQC (version 0.11.5)