FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914212
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8450.052.367361
GAGCAAA61450.048.164361
AGCGAAA17100.040.300931
CGAAAGC17900.038.857633
GCGAAAG18600.037.0171472
TATATCG1656.060509E-835.127065
GTTCGAC1301.6596703E-533.4503142
ACTCCGA7450.033.1062661
GCAAAAG92150.031.7646543
GCTATAT6750.031.166051
CAAAAGC97650.030.714894
GTCGGCT8400.029.3253276
GCCGACC3503.6379788E-1226.92145
AAAGCAG127350.026.7388136
AAGCAGG131450.025.630117
AGTCGGC9700.025.3946935
AGCAAAA116050.025.3555582
GTCGTCT3151.0404619E-925.309402145
CGTATGC2101.5639738E-524.1515128
TCGGCTG10400.023.6866557
Produced by FastQC (version 0.11.5)