FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903484
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC8700.052.4584853
AGCGAAA8800.051.9333081
GCGAAAG9400.048.57882
GAGCAAA40800.033.2483061
GAGCGAA5900.033.1970751
CAAAAGC43300.030.1147354
GCAAAAG49650.026.1172983
GTATATA17200.023.6182461
TACACCG2003.1211076E-421.7328015
AGCAAAA62050.021.4936472
AAAGCAG81000.018.7814336
ACGGTTC3101.4772844E-518.6958668
GTGTCGG4705.153197E-918.50767145
GTAGCCG4557.209019E-817.5149929
GTATTAT23150.017.2345431
AAAAGCA76100.017.134935
AAGCAGG92000.016.3783447
GATATAT17650.016.0290571
GTGTAAG10600.015.0558371
GTTACGT3901.04465114E-414.8803611
Produced by FastQC (version 0.11.5)