FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193871
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA70000.036.1690561
CGAAAGC23200.034.9785923
GCGAAAG24050.033.7592542
AGCGAAA24700.032.3076631
CAAAAGC89550.028.315694
GCAAAAG96150.025.8474143
GTTACGT5350.025.7637831
ACTCCGA6050.023.9819451
AAAGCAG130950.022.0195796
AGCAAAA117300.021.0739652
GTATTAT24700.020.8531271
AAGCAGG132500.020.778437
AAAAGCA128300.020.5541445
GTATATA20750.018.8792821
GTGATCG5407.6397555E-1118.7850939
TACGGGG3151.69523E-518.4002847
GCGATAA2000.00789125718.1363471
TACACCG4506.358641E-817.7094385
TTACGTG8050.017.1099072
TATATAC18300.017.0250973
Produced by FastQC (version 0.11.5)