FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521241
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA502.31191E-672.47312
GAGCGAA6950.061.5745471
AGCGAAA11600.056.9006731
GAGCAAA39150.056.3217121
GCGAAAG12300.054.7967342
CGAAAGC13150.051.785433
GCAAAAG41450.051.0344923
CAAAAGC41050.050.649394
AGCAAAA49350.042.000622
AAAAGCA62850.034.119785
AAAGCAG77650.033.3078466
GCGCGAA1255.1414856E-429.0130921
AAGCAGG112100.023.847377
GAGCTAT8150.023.1118936
GCTATTG7700.021.6399848
GAAAGCA35000.021.5263714
TATATAC10250.019.0829453
ATGACGT3502.0151692E-618.628594
TAGTACT4303.7776772E-818.5323184
CGTATGC2359.248493E-418.497088
Produced by FastQC (version 0.11.5)