FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2602_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2602_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297100
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA89600.054.8926931
GCAAAAG102900.046.118143
CGAAAGC31000.046.041593
GCGAAAG31300.045.160642
CAAAAGC107200.044.4696584
AGCGAAA33250.041.6710621
AGCAAAA116050.040.726052
AAAGCAG157150.034.300816
AAAAGCA155850.030.8669645
GAGCGAA24700.027.6072881
TCGTCGA1907.1293853E-626.7262081
GCTATAT8000.025.3898941
AAGCAGG202600.025.2828377
GTATATA15150.023.9414371
TATATAC12950.023.49773
CTATATA9100.023.1007672
ATAATCG2404.38949E-521.1316133
GTATTAT18650.020.615311
TTATCGC1850.005069859319.5808224
TATACCA35900.018.9699445
Produced by FastQC (version 0.11.5)