FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5749243
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12000.053.180611
AGCGAAA21900.049.670561
GCGAAAG23900.045.1759872
CGAAAGC25950.042.4319843
GAGCAAA133350.042.0920261
CAAAAGC154850.036.0689934
ATGTCGA3350.032.47121
GCAAAAG173800.031.4274143
TTACGTG10500.028.2952692
CCGACTA14700.028.089784
ACATGGT34000.027.6986795
GATAGCG8300.027.0708145
GTTACGT12450.026.2116931
CACCGAC16200.025.4964162
AGCAAAA217550.025.248142
AAAGCAG250000.024.9207216
AAAAGCA229100.024.2213485
AAGCAGG258450.024.0502997
GACGACT6050.023.947794
TACGTGT12950.023.494523
Produced by FastQC (version 0.11.5)