FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899916
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA23050.048.1595271
GAGCAAA91100.046.1924861
CGAAAGC24750.044.7912183
GAGCGAA10800.043.6667981
GCGAAAG26050.042.851682
TGACGCG1707.8485755E-834.109802145
CAAAAGC129800.032.4860654
GCAAAAG139300.029.9604283
AAGCAGG166650.027.694537
GCCGACC3950.027.525316145
ACTCCGA6950.027.1425271
GTTACGT7000.026.948651
AAAGCAG175600.026.323926
ACATGGT28700.026.2542885
AGCAAAA160050.025.9962712
GCTATAT9000.024.1846871
TACGTGT8000.023.5483253
GTCGGCT10300.023.212996
AAAAGCA182850.023.1005525
GAATCGC3152.8478098E-823.0330331
Produced by FastQC (version 0.11.5)