FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7954077
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG317820.39956867402716867No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG282540.35521406192069804No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC282050.3545980256414415No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA280090.3521338805244153No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT276840.34804792561097914No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA272790.3429561971803894No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC253880.3191822256686728No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT235710.29633859466032325No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG211960.2664796933698278No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA189640.23841861224124433No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT169870.2135634341985877No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA161670.20325425564776406No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC155130.19503205714503394No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC152630.19188901490392915No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA152180.1913232673005303No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC149740.188255658073212No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG143440.18033519162562797No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT140930.17717957721555877No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG135580.17045346681959453No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC135060.16979971403344474No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG128080.1610243400962802No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG126030.15844704545857427No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA125070.15724011723799003No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG124330.156309776734623No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT120500.1514946360212505No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA112710.14170091639796797No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT108700.13665947664323594No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA103040.1295436290093747No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC100890.1268406126820246No Hit
ACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG100040.12577197832004894No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT98570.12392386948227935No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA97570.12266665258583742No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC96410.12120828098596481No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA95710.12032822915845547No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT94330.11859326984136563No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC93870.11801495006900234No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG92180.11589025351401551No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCA92170.1158776813450511No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT90820.11418043853485452No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTAGGG90490.11376555695902868No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC88660.11146485003853998No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT86270.1084601016560438No Hit
GACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAGTGAGC84070.10569422448387161No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG83340.104776456149469No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA82920.10424842505296342No Hit
GCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGGGATTTTAGGGT82470.10368267744956454No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT82360.10354438359095593No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA80940.10175913559800842No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG80770.1015454087256133No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC79810.10033848050502905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA22400.084.2226261
GCAAAAG205250.065.595773
CAAAAGC216700.062.6255074
AGCGAAA41700.062.4686161
CGAAAGC47200.056.1891443
GCGAAAG49250.053.1383632
AGCAGGT215000.051.1959278
GCAGGTA199050.050.6037069
AAGCAGG309550.049.2743577
GTAGAAA76400.046.874952
AGGCTCG9050.042.449413145
TAGAAAC86100.042.1645933
AGCAAAA356400.038.790042
CAGTACG50850.038.762139
TACGGAT67950.037.4476782
ACGGATA68850.037.152163
CAAGCTA41500.036.4897234
CTATGCT137550.035.503544
CGGATAA71700.035.4699334
AAGCTAA43000.035.0492065
Produced by FastQC (version 0.11.5)