FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414620
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15700.053.094462
AGCGAAA17650.048.088621
CGAAAGC18700.044.9405483
GAGCGAA9200.026.0211941
GTATATA24500.022.5034261
GTATACG3602.605324E-618.1359841
TATACTG22350.017.826625
GTATAAG14650.017.8264981
CTATACT13450.017.235014
TCGATAG2650.002067347516.4053179
TCTATAC28050.015.755023
AAAGCAG105300.015.6852095
ACGACCG2850.00336364615.2507165
GAGCAAA54850.015.209761
TAGTACC11200.014.8762574
CTATACC26800.014.3259984
GTATAGA25550.013.9125381
ATATAAC23500.013.8728813
TACATCG5751.0290496E-613.8582585
CAAAAGC54650.013.7856434
Produced by FastQC (version 0.11.5)