FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976060
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA26650.060.1638451
GCGAAAG27250.059.055552
CGAAAGC31500.051.5259483
TGACGCG1307.377821E-944.603786145
CTATAGA14200.029.6333121
AACGTGC13350.026.5945368
GTATACG3657.2759576E-1225.839911
GTTATAT25100.024.5689011
TATACGG4154.5474735E-1122.7075122
ACGTGCC15750.022.5420349
TATATAC17100.022.4578573
TGGAACG15750.021.6208635
GTCTAGG28750.021.1973691
TATACCA29400.021.1937055
ACCGTCG12850.020.8629748
AGACCGT14150.020.4816426
GTATATA13500.020.4216291
TGCCGCG6400.020.385324145
CTAAGCG7950.020.0513733
GCCGATT1850.00505706419.5895145
Produced by FastQC (version 0.11.5)