FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3623704
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12250.058.0436251
GAGCAAA93050.053.4899641
CAAAAGC101650.048.0449144
AGCGAAA21000.047.678691
GCAAAAG107850.045.085743
GCGAAAG22650.044.4876332
CGAAAGC24950.041.5338633
AGCAAAA123850.039.2752722
AAAAGCA155050.031.778845
AAAGCAG182350.030.995296
GTAGAAA35550.026.940081
TTACGTG5300.024.6200712
GTTACGT5400.024.1848451
AAGCAGG242250.023.8397357
TACGTGT6150.021.2096863
AGCAGGT47450.020.9225798
TATATAC25150.019.8814453
GAGCTAT17700.019.2411696
GCAGGGG92450.019.2041919
GCTATAT16150.018.8686711
Produced by FastQC (version 0.11.5)