FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224882
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA84250.054.85761
CAAAAGC110200.041.2887884
AGCGAAA25600.041.09561
GCAAAAG112000.040.1755033
CGAAAGC26750.040.0890433
GCGAAAG26900.039.3420222
GAGCGAA15450.037.5689281
AGCAAAA120050.037.556442
AAAGCAG151650.034.017196
CGCGAAA1000.006082033728.9945342
AAAAGCA159550.028.790775
AAGCAGG186400.027.5199837
TGACGCG1907.1758514E-626.703835145
GTATATA11750.025.9345471
GTTACGT2951.4189936E-824.594911
ACTCCGA5650.023.1148621
ACATGGT19300.022.8998855
TATATCG2404.3882854E-521.1323415
GTATAAG5950.019.5105861
TATACTG19350.019.0963345
Produced by FastQC (version 0.11.5)