FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2873795
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7800.053.945481
GAGCAAA68850.051.842131
CAAAAGC75750.046.6749844
AGCGAAA15550.046.6542631
GCGAAAG16950.044.05032
CGAAAGC17750.043.675713
GCAAAAG80750.042.9781343
AGCAAAA100900.034.413252
AAAAGCA116050.030.7780595
AAAGCAG131200.030.2070336
AGTACGG1606.864567E-527.1688165
CGACGGC1400.001001186325.887224145
AAGCAGG161200.025.0802177
GCCGACC3301.7807906E-924.161411145
CCGACGC3851.05592335E-820.70978145
AGCGTGG10850.020.701858
GCTATAT12800.019.8371721
TATATAC22450.019.686483
AACGTGC4904.1654857E-1019.23051145
GCGTGGA11700.019.197879
Produced by FastQC (version 0.11.5)