FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2601_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2601_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218706
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA100800.051.0263671
GAGCGAA14350.048.5320131
AGCGAAA29450.038.181711
CAAAAGC136600.037.6573754
GCGAAAG31200.037.1744542
CGAAAGC31500.036.8003353
GCAAAAG147850.034.2039683
GTTACGT5150.032.3988461
AAAGCAG196900.029.5490196
AGCAAAA172400.029.0969892
AAGCAGG207550.027.8585557
AAAAGCA197150.026.1294735
GTCGAAT2507.853669E-826.0964682
ACATGGT26850.023.2066255
GATAGCG5600.022.004906145
GCATGTT44550.020.8163534
TTACGTG8300.020.0876432
GTTATGT33750.019.9902461
GCGATTA3651.3653698E-719.859383145
CTACTGA42100.019.6198656
Produced by FastQC (version 0.11.5)