FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981175
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6950.065.773511
GAGCAAA54650.041.4247361
GCCGACC2500.037.691776145
AGCGAAA12550.037.5807081
GCGAAAG14300.033.4564822
TATCGTG2507.094059E-1131.880747
CAAAAGC72800.031.548724
CGAAAGC15600.030.6540433
TATATCG2853.255991E-1027.9646225
GCAAAAG79600.027.7622833
ATATGTC26250.024.2892725
AAAGCAG116000.024.1096236
TGACGCG1851.8345611E-423.508387145
TAGATGT23550.023.3817234
AAGCAGG130700.021.5650127
ATCGTGG4151.1023076E-920.9518998
GCTATAT13050.020.5724741
GTATTAT38400.020.4074081
GGCATAC21450.019.9303518
AAAAGCA117600.019.8385375
Produced by FastQC (version 0.11.5)