FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212006
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA70050.049.187081
GAGCGAA9800.046.6315771
CGAAAGC19850.037.5989343
AGCGAAA19250.037.305261
ACATGGT28800.036.9842575
GCGAAAG20400.036.2418942
CAAAAGC107250.031.4147934
ACTCCGA7100.030.6498581
TTATCGC2401.5879777E-930.2015742
GCAAAAG109650.030.1999633
GGTCCTA45750.028.9844869
AAGCAGG132750.028.8744167
GTCACGT7800.027.8992311
AAAGCAG139600.027.5613946
GCCGACC3204.1836756E-1127.182264145
GACATGG40600.026.9481834
GTTGACA39900.026.9063261
GTCGTCT2456.459777E-826.627523145
GCGATTA2207.507242E-726.358559145
TATCGCT2756.8175723E-926.3491233
Produced by FastQC (version 0.11.5)