FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804198
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACACTGTGGTTTTACACAATCTTTTAGCTAAGTCTATTGAAAGTA44520.11702860892098675No Hit
CTCTAGGATTAAACTGAAATGTTTTCTTACATTTGTCCCCACTATGTCCC44000.11566169794526994No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA7300.060.618581
TATCGTG4250.051.147317
GAGCAAA71400.050.8008081
AGCGAAA12950.047.615461
GCGAAAG13750.045.3386882
TTATCGC2000.043.4935072
CCGACGC4700.041.641075145
TATATCG4900.039.925695
ATCGTGG5850.038.3979238
CAAAAGC95950.036.548374
GCAAAAG99300.035.3901943
CGAAAGC17700.035.2059173
GCCGACC2700.034.900818145
TATCGCT2653.6379788E-1232.811483
TAGTACT18500.027.8070074
CTAGTAC8150.026.671913
TTACGTG3306.184564E-1126.3597012
GGTCCTA30450.025.939649
GACATGG25050.025.4532094
AAAGCAG156850.025.31556
Produced by FastQC (version 0.11.5)