FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153751
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA59850.048.7167631
GAGCGAA9250.045.478151
AGCGAAA18250.040.1397861
CGAAAGC18150.039.9163443
GCGAAAG18800.039.32072
TATATCG2900.032.475225
GTCGGCT5850.029.7209076
GCAAAAG97900.029.6008833
CAAAAGC102700.028.5687544
TTATCGC2105.0082053E-727.608932
ACATGGT22250.027.024325
CCGACGC4550.025.487997145
GGTCCTA33250.024.4047539
AAAGCAG141450.023.3543936
AAGCAGG140300.023.2887547
AGCAAAA126900.022.8442692
GTATATA20550.022.2354161
CGATGTT6200.022.20085
GTATTAT31050.021.9574951
TATCGCT3304.733738E-821.9539893
Produced by FastQC (version 0.11.5)