FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4134561
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT58810.1422400104872077No Hit
ATTATATATTGCTTAATTTTTCAGATGTGCCAATGATTTTACAAAATGCT45080.10903213182729679No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT42340.1024050679141026No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11150.051.390611
TATATCG5700.049.5715565
GCGAAAG12150.047.1261252
GAGCGAA6050.045.5574871
ATCGTGG6600.045.008918
TATCGTG6600.043.9111337
CGAAAGC13700.042.3110923
GAGCAAA69300.036.5278171
CGAAGCG1000.006094680628.9823979
CAAAAGC95450.026.7942494
ATATGTC46900.026.5704215
TAGATGT34800.025.815784
TTACGTG4550.025.4870452
TCGTGGT11250.025.1180789
GTCGAAT1751.259156E-424.8498692
CGAAGTG6850.024.3271146
ACATGGT26500.024.0591145
TTATATC20250.023.9713554
AAGGTCG3052.05182E-823.7560639
TATATTG39050.022.8205915
Produced by FastQC (version 0.11.5)