FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472323
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA10900.045.926151
GAGCAAA74050.043.9904861
AGCGAAA18050.042.6055451
CGAAAGC18950.041.672923
GCGAAAG20200.038.3939672
ACTCCGA4800.033.2520871
ACATGGT25850.030.8278855
CAAAAGC105100.030.3283674
TATATCG3400.029.8305135
AAGCAGG133350.027.6526247
GTATTAT34100.027.44561
GCTATAT8750.027.3617151
GCAAAAG117000.026.9983523
TGACGTA2601.1548218E-725.07735
AAAGCAG149800.024.4225586
CAGTACG3001.7214916E-824.151649
GTTGACA34950.023.664381
GGTTATA10700.022.3752351
GTAGCAT44200.022.3230781
CTATATA12750.022.1709842
Produced by FastQC (version 0.11.5)