FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473266
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA11650.059.1786461
GCGAAAG11950.057.0268062
CGAAAGC14000.049.685993
TCGAATA3402.5156623E-923.450592145
GTATAAT17000.023.052191
GAGCGAA6300.023.038641
TATAGTA9900.021.9687422
CCGACGC5201.8189894E-1220.908745145
TATACTG17850.020.7024975
GTATAGG9950.020.422191
TATAGCG2659.3599E-519.150112
TATATAC15750.017.9421633
CATACGC4054.0900522E-717.8910473
TTATACT17450.017.854444
ATTATAC17950.017.7614733
CATACCG2450.001222270417.7449975
CCGTACT3804.40821E-617.1626689
GTATATA15400.016.9648151
CCTATAC11600.016.8653963
TATTGCG3905.6491353E-616.7212495
Produced by FastQC (version 0.11.5)