FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d12_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d12_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254829
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG21900.049.6430322
CGAAAGC24400.045.128993
AGCGAAA25450.042.759591
TACGAAC5500.028.9799449
TATACGA5650.028.2089087
TATAGCG4256.184564E-1122.1699942
TATAATA24900.021.2488122
CTATATA17600.021.0023862
GTATAAT21000.020.382751
GAGCGAA15650.019.006391
GCTATAT17950.018.9960581
GTGATCG6500.018.9484259
ATATACG8050.018.898636
TATATAC25100.018.7603743
GTATAAG12100.017.9872991
TATACCA33500.017.5158755
TAATAGT18700.017.432414
ATATACC22550.017.0261254
TAGCGGT4751.1800148E-716.7758924
GTATATA25350.016.3127421
Produced by FastQC (version 0.11.5)