FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594108
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAATGGCATACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAA25990.10018858120016591No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6750.075.2567141
GAGCAAA54000.051.335831
AGCGAAA14350.047.030761
GCGAAAG16150.042.634942
CAAAAGC67100.040.4845124
GCAAAAG69150.039.1825833
CGAAAGC18500.037.9851343
CGTATGC1406.653827E-736.2229358
AAAGCAG108500.030.1796366
GCCGACC2951.4551915E-1129.483147145
TATATCG2259.17802E-725.7580365
AGCAAAA108600.025.2943762
ACATGGT14650.025.219585
AAAAGCA112300.024.9007725
GTTGACA16300.024.4864641
AAGCAGG132450.024.2853667
TATCGTG3052.0540028E-823.7527437
GCTATAT9250.023.5358851
GTATTGA9700.022.4440121
GACGAAC2953.8899452E-722.11236145
Produced by FastQC (version 0.11.5)