FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2599_d10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2599_d10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339461
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA82950.059.128291
GAGCGAA15900.053.3892361
TATATCG2050.045.9441035
CAAAAGC108650.044.943874
GCAAAAG111400.043.7075963
AGCGAAA27050.039.428941
CGAAAGC27650.038.7828253
GCGAAAG28450.037.4538542
GCTATAT8000.037.184241
ACTCCGA5600.036.27731
AAAGCAG156350.036.2367446
AGCAAAA138500.035.3800472
ACATGGT27600.034.6501545
AAGCAGG182100.031.2320167
TTATCGC2901.2732926E-1129.9794434
GTTGACA32500.029.6915761
AAAAGCA164600.029.4907155
TATCGCT3153.45608E-1127.6001225
CTATATA12650.027.5050322
TGACGCG1751.2587308E-424.850899145
Produced by FastQC (version 0.11.5)